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KMID : 1134820180470100966
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Journal of the Korean Society of Food Science and Nutrition
2018 Volume.47 No. 10 p.966 ~ p.972
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Anti-Tumor Activity of Procyanidin and Its Effect on Hemocytes and Immunity
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Shin Yu-Jin
Lee Je-Hyung
Lee Yong-Kyu
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Abstract
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We investigated the antitumor and immune regulatory responses of mice bearing tumors following treatment with procyanidin. Studies of apoptotic effects and molecular targets of procyanidin based on in vitro experiments have been reported, but few in vivo investigations have been conducted. Therefore, we evaluated the effects of procyanidin on tumor growth, lymphocyte proliferation and immune response in mice bearing P388D1 induced tumors after procyanidin treatment for 21 days. Procyanidin treatment inhibited proliferation of human breast, colon, lymphoma and hepatocarcinoma cancer cells, with IC50 values of 48.5∼63.8 μg/mL being observed. Oral administration of procyanidin (200 mg/kg) or Glivec (10 mg/kg) for 21 days, resulted in tumor inhibition ratios of the procyanidin group (P388D1+procyanidin) and Glivec group (P388D1+Glivec) of 12.89% and 21.22%, respectively. Hemocyte analysis revealed that procyanidin decreased significantly white blood cell (WBC) counts and neutrophil% were significantly increased in the control group (P388D1+CMC), and significantly increased lymphocyte%, red blood cell counts, and hemoglobin levels were significantly decreased in the control group. When the effects of procyanidin on lymphocyte proliferation activity were measured, the procyanidin or Glivec groups showed increased levels compared to the control group. Based on these results, procyanidin is considered to have antitumor activity in vitro and in vivo studies. Moreover, procyanidin treatment restored the increased or decreased hemocyte changes induced in tumor bearing mice to some extent relative to the original state. Procyanidin treatment also enhanced the decreased immune function induced in tumor bearing mice as indicated by lymphocyte proliferation of the procyanidin group being increased significantly compared to the control group. Taken together, these findings indicate that procyanidin plays a crucial role in immunity regulation and tumor inhibitory activity in tumor bearing mice.
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KEYWORD
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procyanidin, anti-tumor activity, immune response, BDF1 mouse
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